American Society of Hematology

Fig. 6.

$Fig. 6. Sensitivity of oligonucleotide primer-probe combinations used in RT-PCR analysis. / RT-PCR was performed on RNA extracted from mixtures of EBV− CA46 cells and serial 10-fold dilutions (10−2 to 10−6) of the virus-productive B95.8 cells. In the case of EBNA1, Rael cells were used instead of B95.8 cells. RNA was extracted from a total of 20 × 106 cells, and a fraction corresponding to 2 × 106 cells was used for each RT-PCR followed by Southern hybridization.$

Sensitivity of oligonucleotide primer-probe combinations used in RT-PCR analysis.

RT-PCR was performed on RNA extracted from mixtures of EBV⁻ CA46 cells and serial 10-fold dilutions (10⁻² to 10⁻⁶) of the virus-productive B95.8 cells. In the case of EBNA1, Rael cells were used instead of B95.8 cells. RNA was extracted from a total of 20 × 10⁶ cells, and a fraction corresponding to 2 × 10⁶ cells was used for each RT-PCR followed by Southern hybridization.

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