Fig. 5.
In vivo limiting dilution analysis of T+KSLA cells.
Five, 7, and 10 T+ KSLA cells were transplanted into irradiated mice together with 2 × 105 bone marrow cells. Peripheral blood cells of the recipient mice were analyzed for engraftment of transplanted cells 12 weeks after transplantation. Reconstitution was observed in 4 of 10 mice with transplants of 5 cells, 5 of 8 mice with transplants of 7 cells, and 6 of 8 mice with transplants of 10 cells. The percentages of negative mice were plotted against the number of injected T+ KSLA cells.
