ETO enhances PLZF/RARα-mediated repression of a retinoic acid response element.

293T cells were transiently transfected with a DR5-RARE-tk-Luc reporter construct along with a tk-renilla internal control plasmid. Results are expressed as fold repression relative to the normalized basal expression of Luc by the pSG5 expression vector. Lane 1, 800 ng pSG5. Lane 2, 800 ng pSG5 and 600 ng ETO. Lane 3, 800 ng RARα. Lane 4, 800 ng RARα and 600 ng ETO. Lane 5, 800 ng PLZF/RARα. Lane 6, 800 ng PLZF/RARα and 600 ng ETO. Lane 7, 800 ng PML/RARα. Lane 8, 800 ng PML/RARα and 600 ng ETO.