PLZF/RARα interacts with ETO. PLZF/RARα and ETO (750 ng) were both transiently transfected in 293T cells grown in 6-well dishes.

Cells were lysed and subjected to immunoprecipitations and immunoblotting. (A) Immunoblot with RARα rabbit polyclonal antibody. (B) Immunoblot with PLZF mouse monoclonal antibody. (C) Immunoblot with ETO rabbit polyclonal antibody. The lanes of all 3 panels correspond to similar experiments. Lane 1, direct immunoblot of cell lysate. Lanes 2-6, extracts immunoprecipitated with mouse control antibody, rabbit control antibody, PLZF antibody, RARα antibody, and ETO antibody, respectively.