Fig. 4.
![Fig. 4. Formation of [32P]PA in [32P]orthophosphate-labeled platelets stimulated with thrombin or collagen. / Platelets labeled with [32P]orthophosphate were incubated without (−) or with 1 U/mL of thrombin (Thr), 20 μg/mL of collagen (Col), or 5 μmol/L Sph for 5 minutes. The lipids were extracted from these platelets, and the lower chloroform phase under alkaline conditions was analyzed for [32P]PA formation.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/96/10/10.1182_blood.v96.10.3431/5/h82200364004.jpeg?Expires=1730503913&Signature=TXVLqEn4sJIiGRGFIm68cMnEirqUJABrYCtOM-jrzFueu7XwRlUaS-FGx6ST3PKI0WC11wSqfHgm0MG8H5XO1gp4dNJMLnWlQHrVnQgDcjMZXWRKrhyZePWXktXnCrij6PAoFcrbXa-K8p8LgWQgsmoY4ARC8qacZzulLsjkWOw9ZzCfdVbQGo4jlRAUIwEVnJ5jpj6hGGdhEikLa6JVTmnW3NCqx5BkANtAtreZNqW4atdo~V7KXZ9oCqYLi5essfWntKrS5i0DlbndE07jq8uMlcyxrZkUmaHLgUR-PivXBRzQlfYBR3YochhdaQ79cCKlom9zP3uoXnSbd7FbjA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Formation of [32P]PA in [32P]orthophosphate-labeled platelets stimulated with thrombin or collagen.
Platelets labeled with [32P]orthophosphate were incubated without (−) or with 1 U/mL of thrombin (Thr), 20 μg/mL of collagen (Col), or 5 μmol/L Sph for 5 minutes. The lipids were extracted from these platelets, and the lower chloroform phase under alkaline conditions was analyzed for [32P]PA formation.
