Fig. 3.
![Fig. 3. Detection of [32P]lysophospholipids in [32P]orthophosphate-labeled platelets. / Human platelets labeled with [32P]orthophosphate were incubated without (−) or with 1 U/mL of thrombin (Thr), 20 μg/mL of collagen (Col), or 5, 10, or 20 μmol/L Sph for 5 minutes. Sph-1-P and LPA were first extracted from these platelets into the upper aqueous phase under alkaline conditions, followed by re-extraction into the lower chloroform phase under acidic conditions. The final chloroform phase was analyzed by TLC autoradiography.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/96/10/10.1182_blood.v96.10.3431/5/h82200364003.jpeg?Expires=1730503988&Signature=mpNfVwqD0TcU3rMwyomPuLFYrrEVDCASacseEI0aSlwUEagZ6ks4O11fPklb37GdMQnnaxtIdaNec99R-oC~rywj8eu68oxGFhozs3RVBRNB5XxJb6oHtIHy8svjjbZRLM4d2goBTzP0K-za0ThE71-ejnqB4AISS~zsmiy7dY0rsHCXy9jhmjxwqqCOR~urLjkokuXqXGXVTE9m9HQotGqWQ4uuNHRkNeMbguGbyQgDJLPC~lEhzRZu7a23nnApAeDkdhCEwOOcQQwern5tlGsowWP7DN8gpEF5c1LA-3S7rh99SlCZGCUi1gnuIvWUgR-bnfCDUQ3ek0jbA0IkYA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Detection of [32P]lysophospholipids in [32P]orthophosphate-labeled platelets.
Human platelets labeled with [32P]orthophosphate were incubated without (−) or with 1 U/mL of thrombin (Thr), 20 μg/mL of collagen (Col), or 5, 10, or 20 μmol/L Sph for 5 minutes. Sph-1-P and LPA were first extracted from these platelets into the upper aqueous phase under alkaline conditions, followed by re-extraction into the lower chloroform phase under acidic conditions. The final chloroform phase was analyzed by TLC autoradiography.
