RCC of A31 and BCL₁ using isolated murine PMN.

Chromium-labeled target cells were mixed with BsAb of varying specificity (as indicated) at 1 μg/mL, and then murine PMN were added at an E:T ratio of 50:1. PMN isolated from huCD64 Tg animals either without (solid bars) or with G-CSF priming (hatched bars) were compared to PMN from huCD64-non Tg littermates, again either unprimed (open bars) or G-CSF treated (lined bars). BsAb were of the specificity indicated. Control BsAb contained the alternative, nonbinding anti-Id Fab' arm for each tumor (ie, anti-BCL₁ for A31 and anti-A31 for BCL₁) and were ineffective. Similar results were obtained in 2 separate experiments. All determinations were performed in triplicate. Error bars represent the SD of the triplicates. ■, nonTg −G-CSF; ▨, nonTg +G-CSF; ▪, Tg −G-CSF; ⊠, Tg +G-CSF.