Microsphere attachment in TNFα stimulated venules.

Attachment of differently coated microspheres was observed in venules stimulated with TNFα for 2.5 hours. Attachment efficiencies were quantified for wild-type (WT) mice, P-selectin knockout mice (P−/−), and E-selectin knockout mice (E−/−) and for mice treated with mAb against E-selectin (10E6, 10 μg), P-selectin (RB40.34, 10 μg), and neutrophils (RB6-8C5 10 μg). Data are presented as mean ± SE. **Denotes significant difference (P < .01) from PSGL-1(293/FTIII) in WT mice with no mAb.