Fig. 8.
Fig. 8. Effect of M-CSF on SP (CD7−45RA+) and DP (CD7 + 45RA+) HPC growth and differentiation. / Cells from cultures under the standard condition, with (+) or without (−) M-CSF, were recovered on day 12, labeled, and analyzed as indicated in the legend of Figure 6. (A) Data (means of 3 experiments) are expressed as absolute labeled cell numbers ([percent labeled cells × cell number]/100) normalized relative to 1 × 105 seeded HPCs; statistical significance of differences between the (+) and (−) conditions are indicated (upper panel). (B) Results of one experiment are shown as 2-color fluorescence plots of cells labeled with CD1a-FITC and CD14-PE mAbs; quadrants were set according to control antibody labeling.

Effect of M-CSF on SP (CD745RA+) and DP (CD7 + 45RA+) HPC growth and differentiation.

Cells from cultures under the standard condition, with (+) or without (−) M-CSF, were recovered on day 12, labeled, and analyzed as indicated in the legend of Figure 6. (A) Data (means of 3 experiments) are expressed as absolute labeled cell numbers ([percent labeled cells × cell number]/100) normalized relative to 1 × 105 seeded HPCs; statistical significance of differences between the (+) and (−) conditions are indicated (upper panel). (B) Results of one experiment are shown as 2-color fluorescence plots of cells labeled with CD1a-FITC and CD14-PE mAbs; quadrants were set according to control antibody labeling.

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