Differentiation of NK cells from CD34⁺CD7⁺CD45RA⁻, CD34⁺CD7⁺CD45RA⁺, and CD34⁺CD7⁻CD45RA⁺ HPCs.

Cells were cultured for 3 weeks with SCF/IL-2/IL-7/IL-15, labeled with CD8-FITC and CD56-PE mAbs, and FACS analyzed. Quadrants were set based on labeling with control mAbs. CD56⁺CD8⁻ NK cell percentages, indicated in the upper left corner, are from one representative experiment of 5 for bulk (Bk) CD34⁺ HPCs, CD34⁺CD7⁺CD45RA⁻(CD7⁺45RA⁻) and CD34⁺CD7⁻CD45RA⁺(CD7⁻45RA⁺) HPCs, and of 7 for CD34⁺CD7⁺CD45RA⁺(CD7⁺45RA⁺) HPCs.