The κB site that overlaps with the STAT3 binding site is required for IL-1β inhibition.

(A) Diagrams of constructs F3, M1, and M3 show the relative position and modifications of the STAT3 and NF-κB binding sites. (B) Each construct was transfected into rat hepatoma H4IIE cells to test for its IL-6 response. (C) Each construct was transfected into human hepatoma HepG2 cells to test for the IL-1β-inhibitory function. The results presented are the means of fold induction above the control levels from triplicate experiments. The error bars indicate the standard deviations.