![Fig. 4. Platelet Ca++ mobilization induced by CRP requires SLP-76 and is delayed by PI 3-kinase inhibition. / The roles of SLP-76 and PI 3-kinase on PLC-γ2 tyrosine phosphorylation and Ca++ mobilization were studied in SLP-76–deficient mouse platelets (−/−) (upper panels) and in human platelets preincubated for 15 minutes with 50 nmol/L wortmannin (+Wort.) (lower panels). (A, D) Platelets were stimulated with 3 μg/mL CRP for 2 minutes. PLC-γ2 immunoprecipitates were resolved by 8% SDS-PAGE and probed with a 1:1 mixture of 4G10 and PY20 anti-phosphotyrosine antibodies. Equal loading of PLC-γ2 was verified by Western blotting (not shown). Results shown are representative of 2 experiments. Indo1-am–preloaded platelets were stimulated for 3 minutes with either 3 μg/mL CRP (B, E) or 1 U/mL thrombin (C, F). [Ca++]i was calculated as described in the experimental procedures section. Results shown are representative of 3 experiments.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/96/12/10.1182_blood.v96.12.3786/5/h82300406004.jpeg?Expires=1738170099&Signature=CTWr7yu6oygQxi7M7LzgIrfbV1J90~VHRtAI2WAKQaH0PORICT4enFlqTNZTLv-UGPGNBx9oPh5BXR-mQCFWcpjKVCFBEu3C4cXJ1JLdpxmQ6NFLFIJrtbJrEJA62ahhlPDFF6fFc5SqL9kkv70ytosf23gmgVy6avk9omGAatPXEV3hBviJhqy-BlfzYDtm-4HLDngoF8A2lmAzn1CiZH5uOGAtg5mnkaKZUo~mXX8NVUIo63Z~Q7Q8MTX~O-195XKuScn7FHKgegqGWQUzmRQXhj2ww82PrAbv6qlU79566961P6f-wtAFlK8PODzvkXE4d~nG7u2cFn5rvDLgRw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Platelet Ca++ mobilization induced by CRP requires SLP-76 and is delayed by PI 3-kinase inhibition.
The roles of SLP-76 and PI 3-kinase on PLC-γ2 tyrosine phosphorylation and Ca++ mobilization were studied in SLP-76–deficient mouse platelets (−/−) (upper panels) and in human platelets preincubated for 15 minutes with 50 nmol/L wortmannin (+Wort.) (lower panels). (A, D) Platelets were stimulated with 3 μg/mL CRP for 2 minutes. PLC-γ2 immunoprecipitates were resolved by 8% SDS-PAGE and probed with a 1:1 mixture of 4G10 and PY20 anti-phosphotyrosine antibodies. Equal loading of PLC-γ2 was verified by Western blotting (not shown). Results shown are representative of 2 experiments. Indo1-am–preloaded platelets were stimulated for 3 minutes with either 3 μg/mL CRP (B, E) or 1 U/mL thrombin (C, F). [Ca++]i was calculated as described in the experimental procedures section. Results shown are representative of 3 experiments.
