RT-PCR amplification of RNA from mice.

RNA was isolated from the bone marrow or spleen cell suspensions of transplanted or nontransplanted mice as indicated. The cDNA was prepared and analyzed for the presence of transcripts for human KDR or human proline hydroxylase as described in “Patients, materials, and methods.” The PCR products were separated on agarose gels and visualized with ethidium bromide. Negative control, mock PCR using water instead of cDNA; positive control, cDNA from human umbilical vein endothelial cells (HUVECs) for KDR, or KMST-6 fibroblasts of human Dexter cultures for proline hydroxylase. CD45⁺CD14⁺: analysis of sorted cells from Dexter cultures as described in “Patients, materials, and methods.” A total of 3 of 9 transplanted mice were positive for human KDR cDNA and 6 of 8 mice for proline hydroxylase. BMC indicates analysis from a bone marrow culture established from the bone marrow of a mouse transplanted with CD34⁺ PBCs.