Transduced MO7e-G cells express the G-CSF receptor and show synergistic proliferation in response to SCF and G-CSF.

(A) Three days after infection, MO7e cells transduced with pMFG-G-CSF receptor retroviral concentrate (MO7e-G cells) were stained with either antihuman G-CSF receptor antibody (filled) or an idiotype control (unfilled), and fluorescein isothiocyanate–conjugated secondary antimouse antibody, and analyzed by flow cytometry. One experiment representative of 4 is presented, in which 94% of the cells express the receptor. Untransfected wild-type MO7e cells did not express the G-CSF receptor (dotted line). (B) Parental MO7e cells and MO7e-G cells were factor starved for 18 hours and then incubated in medium containing no growth factor, 100 ng/mL SCF, 10 ng/mL G-CSF, or both. The data presented represent the means and SD of 4 independent experiments.