Fig. 6.
Effect of mocarhagin on ristocetin-induced platelet aggregation in mGPIb−/−;TghGPIb mice.
Ristocetin is an antibiotic that causes platelet aggregation of human but not mouse platelets in the presence of human vWF.25Washed platelets (3 × 108) from WT mice (A) or mGPIb−/−;TghGPIb mice (B) were treated under stirring conditions (1000 rpm) at 37°C in a lumi-aggregometer, with 1.5 mg/mL ristocetin and 5 μg/mL multimeric human vWF. (C) Washed mGPIb−/−;TghGPIb platelets were incubated with 10 μg/mL mocarhagin before ristocetin treatment. Aggregation was followed for 2 minutes.
