GFP expression in multiple lineages.

FACS analysis of 1 mouse transplanted with cells transduced with the lentivirus-GFP in our 96-hour with cytokines protocol. Peripheral blood was collected 16 weeks after transplant and stained with an isotype-PE, B-220-PE, Mac-1-PE, or CD3-PE. Gates were set for GFP, using an untransplanted mouse and for lineage by isotype staining. Dead cells were excluded with propidium iodide.