HPLC profile of lysylendopeptidase digests of Bβ chain.

The lysylendopeptidase digests of Pe-Bβ chains derived from normal fibrinogen and fibrinogen Osaka VI were injected onto a TKS GEL ODS-80 column (4.6 × 150 mm), and the peptides were eluted with a linear gradient from 0% to 40% acetonitrile in 100 minutes. Three Osaka VI peaks, K16, K17, and K34, and normal K16 and K17 were collected and subjected to N-terminal amino acid sequence analysis.