Fig. 4.
Effects of Zn at the indicated doses in Hep3B cells with or without exposure to hypoxia or Co.
(A) EPO protein production and cell viability at 24 hours. (B) EPO mRNA expression at 6 hours. (C) DNA binding activity of hypoxia inducible factor-1 (HIF-1) at 6 hours. (D) Effect of zinc (Zn) (100 μM) on either hypoxia (1% O2), cobalt (Co) (100 μM), or both stimulants on induced EPO protein production in Hep3B cells for 24 hours. Data are expressed as mean ± SD of 3 independent experiments. Experimental conditions were as described in the legend to Figure 1.
