Tyrosine phosphorylation of PLC-γ1 in response to PDGF-BB alone (control), FVIIa, or FFR-FVIIa in combination with PDGF-BB.

Cells were incubated with 100 nmol/L FVIIa or FFR-FVIIa for 1 hour and then with or without PDGF-BB at indicated concentrations. Cell were lysed, and tyrosine phosphorylation of PLC-γ1 was detected as described in “Materials and methods.”