Fig. 2.
ATRA induction of FR-β in AML blasts from patient marrow.
Cells (1 × 106) were cultured and treated with or without ATRA (1 μmol/L) for 6 hours (panels A and B), 24 hours (panel C), or 5 days (panel D). The samples were obtained from separate patients with FAB-M2 (panels A and C) or FAB-M4 (panels B and D) type AML. Cell lysates were then examined by Western blot with the use of rabbit anti–FR-β and AP-conjugated secondary antibody.
