Fig. 1.
Megakaryocytic colony formation in culture by ET, 2°T, and healthy circulating progenitor cells as a function of PEG-rHu MGDF concentration.
PBMNCs from an ET patient (upper panels) and a 2°T patient (lower panels) (▴) are compared with those of healthy volunteers (▪) with respect to the numbers of megakaryocytic colonies (mean ± SEM) of quintuplicate samples (A) and the percentage maximum of megakaryocytic colonies (B) produced at various concentrations of PEG-rHu MGDF. The cell number plated in microwells (upper panels) was 4 × 104 PBMNCs per 0.1 mL culture (experiment 218). The cell number plated in mini-macro wells (lower panels) was 1.5 × 105 cells per 0.7 mL culture (experiment 188).
