Effects of STI571 on survival after ionizing radiation.
Bone marrow cells from BALB/c mice were treated with 0, 0.1, or 1 μmol/L STI571 for 24 hours before being exposed to 0-6 Gy gamma irradiation. After irradiation, cells (5 × 104 to 2 × 105 cells) were plated in triplicate in 1 mL Iscove modified Dulbecco medium containing 1% methylcellulose, 20% fetal bovine serum, 5% WEHI-3B conditioned medium as a source of murine IL-3, 20 ng/mL recombinant human G-CSF, and STI571 (at the same concentration as pretreatment). (A) CFU-GMs (> 50 cells) were enumerated on day 8 to determine radiation response. The results are presented as the percentage of surviving colonies relative to an unirradiated control. (B) U937 cells were treated with 0, 0.1, or 1 μmol/L STI571 for 24 hours before being exposed to 0- 6 Gy gamma irradiation. After irradiation, cells (1 × 103 cells) were plated in 1 mL of RPMI 1640 containing 1% methylcellulose, 20% FCS, and STI571 (the same concentrations as pretreatment). Colonies were counted on day 8. Shown here is 1 of 2 independent experiments.
