IFN-γ induced decrease in the fusion of MDM with M-tropic envelopes and reduction in the surface CCR5.

(A) Fusion was set up between MDMs cultured in the absence or in the presence of IFN-γ and effector 12E1 cells infected with recombinant vaccinia virus expressing M-tropic envelope (vCB28, JR-FL). Syncytia were scored after 18 hours. (B) The same MDMs were stained with anti-CCR5 2D7 monoclonal antibody. The ΔMFC was calculated as described in Figure 4. The results represent 11 separate experiments.