Effect of IFN-γ on the in vitro infection of macrophages with M-tropic HIV-1.

(A) PCR-based analyses of viral DNA from MDMs infected with M-tropic HIV-1. MDMs untreated or treated with IFN-γ were infected with Ba-L or with ADA for 48 hours. DNA lysates were amplified with gag-specific primers as described in Figure 2. (B) DNA from a serial dilution of ACH-2 cells (containing one proviral copy/cell) was amplified by PCR as internal control. (C) MDMs untreated (▵) or treated with IFN-γ (▴) were infected with Ba-L for 48 hours. Infected MDMs were washed and cultured in the presence of IFN-γ. At indicated time points, the aliquots of the culture medium were assayed for p24. Control cultures included uninfected MDMs (♦). Data shown for 1 experiment are representative of 3 experiments performed with macrophages derived from different individuals.