Flow cytometric analysis of DCs derived from flt3⁺/lineage⁻ cells.

Flt3⁺/lineage⁻ cells were sorted from BM and cultured for 9 days in 200 ng/mL FL plus conditioned media from spleen cells. LPS at 1 μg/mL was added during the last 24 hours to induce maturation. Gates were determined with the use of isotype controls as described in “Materials and methods.” Results shown are representative of 5 experiments.