Fig. 4.
Kinetics of DC development from FL-supplemented C57BL/6 BM cultures.
BM cells were cultured in FL at 1 × 106 cells per milliliter and, on days 3, 5, 7, 9, and 11 of culture, were harvested for enumeration of cells and analyzed for the expression of Gr-1 (▴), CD19 (♦), CD11c (▪), and MHC class II (●). Results are presented as the mean ± SD from 3 separate experiments. Day 0 data were generated from freshly isolated BM cells. Gates for flow cytometric analysis were determined with the use of isotype controls as described in “Materials and methods.” Analysis was performed a minimum of 3 times for each cell-surface marker.
