DC development from BM cells cultured in FL.

DC development from BM cells cultured in FL requires endogenous IL-6, but not GM-CSF. (A) C57BL/6 BM cells were cultured in FL plus anti–mIL-6 (murine IL-6) mAb, anti–mGM-CSF mAb, or control antibodies for 9 days as described in “Materials and methods.” Replicate wells (n = 3) were harvested and scored for cellularity. (B) C57BL/6 or IL-6 gene KO BM cells were cultured in FL plus anti–mIL-6 or control mAb for 9 days as described in “Materials and methods.” Replicate wells (n = 3) were harvested and scored for cellularity and results are presented as mean ± SD. Each experiment was performed a minimum of 3 times. *Significantly different from normal BM cells cultured in FL plus rat mAb isotype control (P < .05).