Effect of Thal and analogs on growth and apoptotic signaling in MM.1S and MM.1R cells.

(A) MM.1S cells were cultured in media, with 50 μmol/L of PD98059 and with 10 μmol/L of IMiD1, IMiD2, or IMiD3 for 48 hours. Cells were then triggered with 50 ng/mL of IL-6 for 10 minutes, lysed, transferred to PVDF membrane, and blotted with anti-phospho MAPK Ab. Blots were stripped and reprobed with anti-ERK2 Ab. (B) MM.1S and MM.1R cells were treated with Thal (100 μM), IMiD1 (100 μmol/L), or Dex (10 μmol/L) and harvested at 12 hours. Total cell lysates were subjected to immunoprecipitation with anti-RAFTK Ab and analyzed by immunoblotting with anti-P-Tyr Ab or anti-RAFTK Ab.