Effect of Thal and analogs on p21 expression in MM cell lines and patient cells.

(A) MM.1S cells were cultured with 10 μmol/L of Thal, IMiD1, IMiD2, and IMiD3 for 48 hours. MM.1S cells were also cultured with IL-6 (50 ng/mL) alone and with IMiD1, 10 μmol/L Dex, and Dex plus IL-6. Cells were lysed, subjected to SDS-PAGE, transferred to PVDF membrane, and blotted with anti-p21 Ab. The membrane was stripped and reprobed with anti-α-tubulin Ab. (B) MM.1S, Hs Sultan, and patient MM cells were lysed and immunoprecipitated with wt-p53 and mt-p53 Abs, transferred to PVDF membrane, and blotted with anti-p53 Ab.