SH2 domains of distinct signaling molecules bind to phosphorylated Dok-1.

GST-fusion proteins containing the SH2 domains of several signaling molecules were incubated with lysates of SCF-induced Mo7e cells. As a reference for p62Dok-1, a Tec immunoprecipitate was included. The precipitates were analyzed by Western blot analysis with an antiphosphotyrosine antibody (upper panels). The blots were stained with Ponceau S as a control for the amount of added GST-fusion protein (lower panels). Phosphorylated Dok-1 binds to the SH2 domains of various signaling intermediates.