IFN-γ staining before and after 3 vaccinations.

Intracellular IFN-γ staining in patient no. 10 (upper panel) and patient no. 6 (lower panel) before and after 3 vaccinations. PBMNCs from each patient were incubated with autologous PBMNCs pulsed with the indicated peptides for 6 hours, and IFN-γ production was assessed by flow cytometry after intracellular staining for IFN-γ in CD8 positive lymphocytes. Numbers represent the percentage of IFN-γ–expressing cells in the lymphocyte gate.