Percentages of nonapoptotic and apoptotic erythroblasts in the G1/G0 and S phases of the cell cycle.

Folate-deficient (FDCs) or normal control (NCs) erythroblasts from CD₂F₁ mice were isolated, cultured, processed and analyzed by flow cytometry as described in Figure 1 legend. The percentages of nonapoptotic (TUNEL negative) cells in the G0/G1 phase are shown in panel A and those in the S phase are shown in panel B. The percentages of apoptotic erythroblasts (TUNEL positive) erythroblasts in the G0/G1 phase are shown in panel C and those in the S phase are shown in panel D. FDC-CM, ●; FDC-FDM, ○; NC-CM, ▴; NC-FDM, ▵. Data are ± 1 SEM from 5 separate experiments with FDCs and 3 separate experiments with NCs. *, statistically significant differences in FDC-FDM.