Inhibition of platelet aggregation by TIMP2 and BB94.

(A) Platelets were pretreated for 2 minutes with MMP inhibitors as indicated and then triggered by collagen (1 μg/mL), AA (10 μmol/L), or ADP (10 μmol/L). The figure shows the inhibition by TIMP2 (0.5-1.5 μg/mL) and BB94 (2.5-4 μmol/L) but not by TIMP1 (1.5 μg/mL) of platelet response. Results are representative of 3 separate experiments. (B) The supernatants of platelets treated as indicated on the figure were analyzed by gelatin zymography.