Flow cytometry analysis of MMPs on the platelet membrane surface.

Resting and collagen- (0.5 μg/mL) activated platelets were treated as described in “Materials and methods.” (A) Nonspecific fluorescence intensity where resting (i) and activated (ii) platelets were pretreated with mouse IgG (control isotype). (B-E) Fluorescence intensities when resting (i) and activated (ii) platelets were pretreated with specific MMP9 (B), MMP2 (C), MT1-MMP (D), and TIMP2 (E) monoclonal antibodies, respectively. Results are representative of 3 separate experiments.