Expression of TIMP1 and TIMP2 in human platelets.

Resting and collagen- (1 μg/mL) activated platelets were centrifuged and analyzed for TIMP expression by reverse zymography. Lane 1: molecular weight markers; lane 2: human recombinant TIMP2; lane 3: supernatants from resting platelets; lane 4: supernatants from activated platelets; lane 5: activated platelet pellet; lane 6: purified platelet membranes.