Detection of the MEL1 expression in patient RNA, cell lines, and organs by RT-PCR.

(A) Expression of the MEL1 gene in RNA from patient leukemia cells or cell lines by RT-PCR. Each transcribed cDNA from leukemia cells with t(1;3)(p36;q21) or cell lines as source of RT-PCR was amplified by the MEL1 specific primers (see “Methods and materials”). Lanes are indicated as follows: 1, 1-kb ladder marker (Promega Biotech); 2, negative control (water); 3, control genomic DNA; 4 to 6, leukemia cells with t(1;3)(p36;q21), cases 1 to 310; 7, KG-1; 8, leukemia cells with inv(3); 9, MOLT4; 10, Kasumi-4; 11, CMK; and 12, MEL1 cDNA fragment (N1163) as a positive control. (B) Expression of the MEL1 gene in various organ RNAs by RT-PCR. The MEL1 and EVI1expressions were analyzed in various organs by RT-PCR using each specific primer set.