Physical maps of the chromosomal breakpoints at 3q21 and 1p36 in t(1;3)-positive MDS/AML.

(A) Mapping of the breakpoints at 3q21. Arrows and numbers indicate the breakpoints in cases 1 to 3 with t(1;3)(p36;q21), and arrowheads indicate the breakpoints in 7 AML cases with t(3;3)(q21;q26) or inv(3)(q21q26) (3q21q26 syndrome) previously reported.4-6The position and orientation of the RPN1 gene are indicated by a horizontal arrow. (B) Mapping of the breakpoints at 1p36. Arrows and numbers indicate the breakpoints in the 4 cases analyzed. The positions of the P1 phage clone (P1-106) and BAC clones (40A and 209F) are indicated below. A F11 cDNA fragment was isolated by the exon trapping method. The position and orientation of the MEL1gene are indicated by a horizontal arrow. Restriction sites are indicated by the following letters: B (BssHII), S (SfiI), Nt (NotI), and Nr (NruI).