Presence of CD95L in B-CLL cells.

(A) Reverse transcription–PCR detection of CD95L mRNA. Lanes 1 to 6 correspond to B-CLL cells from different patients. Lane 7 corresponds to Jurkat cells induced by PMA and ionomycin (positive control). GAPDH mRNA was used as mRNA quantity control. (B) Western blot analysis of CD95L expression in B-CLL lymphocytes (lanes 1 to 4). Lanes 5 and 6 correspond to Jurkat cells cultured in the presence (positive control: +) and in the absence (negative control: −) of PMA plus ionomycin. (C) Western blot analysis of the expression of CD95L proteins by nonstimulated (control) and PMA-induced B-CLL cells.