CLHb abolished the inhibitory effect of 3-morpholinosydnonimine (SIN-1) on platelet aggregation.

The effect of o-raffinose CLHb on SIN-1–mediated platelet inhibition was evaluated by using impedance aggregometry on rabbit whole blood. Collagen (1 μg/mL) was used in all experiments. (A) A representative experiment, in which aggregation curvea is that of the control; b, SIN-1 (50 μmol/L);c, CLHb (50 g/L); and d, CLHb and SIN-1. (B) The aggregation amplitudes measured at 6 minutes in 14 experiments. The change in platelet aggregation (Δ aggregation) was calculated as the aggregation in the presence of CLHb minus that observed in Ringer's lactate alone. The data are expressed as means; error bars denote 1 SD. *P = .001 and #P = .005 compared with results with Ringer's lactate alone.