Representative flow cytometric analyses of peptide-specific IFN-γ production by tetramer-binding cells.

PBMC from subjects 307 and 350 were stimulated with the relevant HLA-A2 gag or RT peptide for 8 hours. Brefeldin A (10 μmol/L) was added during the last 4 hours of incubation. Cells were washed and stained with tetramer PE, CD8-Cy5, and IFN-γ FITC. (A) Percentages represent the proportion of tetramer-staining cells within the CD8-gated population. (B, C) IFN-γ–secreting CD8 T cells in the absence or presence, respectively, of cognate peptide.