Effects of heparin and syndecan-1 on HGF-induced IL-11 production by Saos-2 cells.

(A) Saos-2 cells were stimulated with HGF 1 ng/mL and varying concentrations of heparin, and IL-11 concentration in the supernatants were determined by ELISA. Bars represent percentage of control IL-11 production in cells receiving only HGF (1 ng/mL). Results are graphed as mean + SD of 3 experiments. A potentiation of the HGF effect was seen at 10 to 1000 ng/mL of heparin. Higher concentrations of heparin inhibited the HGF effect. Heparin alone had no effect on IL-11 production. (B) Saos-2 cells were stimulated with HGF 1 ng/mL and varying concentrations of purified syndecan-1 (Synd.). IL-11 concentrations in the supernatants were determined by ELISA. Bars represent percentage of control IL-11 production measured in cells receiving only HGF (1 ng/mL). Results are graphed as mean + SD of 4 experiments. A potentiation of the HGF effect was seen at 150 to 1500 ng/mL of syndecan.