Coprecipitation of syndecan-1 and HGF.

Two myeloma cell lines and pleural fluid from 2 patients with myeloma were examined for complexes of syndecan-1 and HGF by coprecipitation. Proteins on the blot were, in all cases, stained with anti-HGF antibodies. Protein was immunoprecipitated with the anti–syndecan-1 antibody BB-4 (lane 1), BB-4 in the presence of heparin (lane 2), and an irrelevant antibody (lane 3). Panels A-E consist of (A) 3 ng of purified HGF as a control, (B) immunoprecipitates from the ANBL-6 cell line, and (C) immunoprecipitates from the JJN-3 cell line. (D) and (E) are immunoprecipitates from patients with myeloma 1 and 2, respectively.