Oxidized LDL-induced tissue factor pathway activity as a function of time and compared with other known agonists at 4 hours in smooth muscle cells.

(A) Oxidized LDL (●) or native LDL (○), 150 μg cholesterol/mL, was added to rat aortic smooth muscle cells at various times before measuring tissue factor pathway activity. (B) Serum and α-thrombin or lipoprotein was added to wells of rat aortic smooth muscle cells at the specified concentrations 4 hours before tissue factor assay. NT indicates no treatment (control). All cells had been in serum-free DMEM for 48 hours at the time of assay. Data represent means ± SD of 4 wells per treatment. Data are from an experiment representative of multiple experiments.