American Society of Hematology

Fig. 4.

$Fig. 4. Effect of IL-8 on the association of CD44 with the cytoskeleton. / HCs were incubated on HA with or without IL-8. Cells were then lysed with Triton, and the lysates were centrifuged. The Triton-insoluble fractions were resuspended in SDS buffer and then run on SDS-PAGE, and protein was transferred to a nitrocellulose membrane and immunoblotted with anti-CD44.$

Effect of IL-8 on the association of CD44 with the cytoskeleton.

HCs were incubated on HA with or without IL-8. Cells were then lysed with Triton, and the lysates were centrifuged. The Triton-insoluble fractions were resuspended in SDS buffer and then run on SDS-PAGE, and protein was transferred to a nitrocellulose membrane and immunoblotted with anti-CD44.

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