Fig. 3.
Fig. 3. Effect of hirudin on thrombin-induced DAF expression and comparison of TRAP-6 to thrombin for induction of DAF. / (A) HUVEC monolayers were treated with hirudin (30 U/mL) or plain medium alone for 30 minutes prior to the addition of thrombin (10 U/mL) and cultured for a further 24 hours. Following harvesting, ECs were analyzed by flow cytometry for the expression of DAF using MoAb 1H4. The data are expressed as a percentage of the DAF expression (RFI) on thrombin-stimulated ECs and are presented as the mean ± SEM. (B) HUVEC monolayers were treated with increasing concentrations of TRAP-6 or vehicle alone for 24 hours and then harvested, stained with MoAb to DAF (1H4), and analyzed by flow cytometry. The data are expressed as a percentage of the DAF expression on thrombin-treated cells and are presented as the mean ± SEM of 3 experiments performed on separate HUVEC cultures.

Effect of hirudin on thrombin-induced DAF expression and comparison of TRAP-6 to thrombin for induction of DAF.

(A) HUVEC monolayers were treated with hirudin (30 U/mL) or plain medium alone for 30 minutes prior to the addition of thrombin (10 U/mL) and cultured for a further 24 hours. Following harvesting, ECs were analyzed by flow cytometry for the expression of DAF using MoAb 1H4. The data are expressed as a percentage of the DAF expression (RFI) on thrombin-stimulated ECs and are presented as the mean ± SEM. (B) HUVEC monolayers were treated with increasing concentrations of TRAP-6 or vehicle alone for 24 hours and then harvested, stained with MoAb to DAF (1H4), and analyzed by flow cytometry. The data are expressed as a percentage of the DAF expression on thrombin-treated cells and are presented as the mean ± SEM of 3 experiments performed on separate HUVEC cultures.

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