Fig. 5.
Ligand-dependent dissociation of the corepressor SMRT from wild-type and mutant PML/RARα fusion proteins on a DR5 RARE in gel mobility shift assay.
Interaction of SMRT with wild-type and mutant PML/RARα S-form (A) and L-form (B). In vitro translated PML/RARα fusion proteins were coincubated with the [32P]-labeled DR5 RARE, along with bacterially expressed GST-SMRT-IDII in the presence of increasing concentrations of RA. The position of the complex shifted by SMRT is indicated.
