Specific nuclear RA-binding activity in Cos-1 cells transfected with wild-type in comparison with those transfected with mutant PML/RARα fusion proteins.

HPLC RA-binding profiles of wild-type (top panels) and mutant (bottom panels) PML/RARα S-form (A) and L-form (B). Nuclear extracts were incubated with 10 nmol/L [³H]-RA or with [³H]-RA in the presence of 200-fold excess of unlabeled RA. Extracts were subjected to HPLC analysis using a 6 HR 10/30 size exclusion column.