p27 immune complex formation during terminal erythroid differentiation.

Whole cell lysates from FVA erythroblasts (0, 24, or 48 hours) were immunoprecipitated with anti-p27 in the absence (−) or presence (+) of blocking peptide. Immune complexes were electrophoretically separated in 12% polyacrylamide gels and transferred to PVDF membranes. Western blot analysis was performed with antibodies to p27, cdk2, cdk4, or cyclin D3.