Immunohistochemical studies detecting LL-37 and α-defensins.

(A) Detection of LL-37 in different lymphocytes by double-staining immunohistochemical analysis. For identification of cell types, the following fluorescein isothiocyanate–conjugated monoclonal antibodies against cell-surface markers were used: CD3 for T cells, γδ chain of T-cell receptor for γδ T cells, CD14 for monocytes/macrophages, CD19 for B cells, and CD56 for NK cells. The LL-37 antibody was detected with a donkey α-rabbit IgG conjugated with Texas red. The cell types appear green; LL-37, red; and doubly stained cells, yellow. (B) Detection of α-defensins in different lymphocytes by double-staining immunohistochemical analysis. The monoclonal antibodies were the same as in A, except for CD3, and the α-defensin antibody was detected with a donkey α-mouse IgG conjugated with indocarbocyanine (red appearance).