Effect of hypoxia on uPA and uPAR mRNA expression.

(A) hMVECs were cultured for 72 hours in normoxic and hypoxic conditions in M199 supplemented with 10% HS and not stimulated (control) or stimulated with 10 ng/mL TNF-α or 10 ng/mL each FGF-2+TNF-α. After 72 hours, total RNA was isolated and analyzed by Northern blotting using α-³²P CTP-labeled probes for uPA, uPAR, and actin. (B) The signals for uPA and uPAR mRNA were quantified by Phosphorimager analysis and adjusted for the corresponding actin mRNA. The data are expressed as a percentage of normoxic control cells. Similar results were obtained in 3 independent experiments.